{"id":8193,"date":"2022-12-12T12:11:00","date_gmt":"2022-12-12T12:11:00","guid":{"rendered":"https:\/\/arrayjet.com\/?p=8193"},"modified":"2023-02-23T12:48:46","modified_gmt":"2023-02-23T12:48:46","slug":"switch-rppa-assay-to-inkjet-printing","status":"publish","type":"post","link":"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing","title":{"rendered":"Application Note: Switch your RPPA Assay to Inkjet Printing"},"content":{"rendered":"\n<p>Many research groups&nbsp;print microarrays of&nbsp;cell lysates and protein extracts for Reverse Phase Protein Array (RPPA).&nbsp;<\/p>\n\n\n\n<p>Our latest&nbsp;application note describes a modified Laemmli buffer formulation and protocol recommendations for RPPA printing on inkjet microarrayers with improved density, spot morphology, and speed.<\/p>\n\n\n\n<p>It is intended as a basic guide for researchers and technicians considering moving their RPPA printing procedures from contact pin spotting to inkjet microarray technology.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Read the full application note:<\/h2>\n\n\n\n<p>RPPA &#8211; Transition from contact pin spotting of cell lysates and protein extracts to high-speed, contactless inkjet printing \/ Modification of Laemmli buffer formulation for Reverse-Phase Protein Array (RPPA) using Arrayjet inkjet microarrayers.<\/p>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div data-wp-interactive=\"\" class=\"wp-block-file\"><object data-wp-bind--hidden=\"!selectors.core.file.hasPdfPreview\" hidden class=\"wp-block-file__embed\" data=\"https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/02\/RPPA-Transition-from-contact-pin-spotting-to-inkjet-printing.pdf\" type=\"application\/pdf\" style=\"width:100%;height:600px\" aria-label=\"Embed of RPPA-Transition-from-contact-pin-spotting-to-inkjet-printing.\"><\/object><a id=\"wp-block-file--media-2e698465-26f9-420b-9576-f7c0a3c8bb4e\" href=\"https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/02\/RPPA-Transition-from-contact-pin-spotting-to-inkjet-printing.pdf\">RPPA-Transition-from-contact-pin-spotting-to-inkjet-printing<\/a><a href=\"https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/02\/RPPA-Transition-from-contact-pin-spotting-to-inkjet-printing.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-2e698465-26f9-420b-9576-f7c0a3c8bb4e\">Download<\/a><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Want to learn more? <\/h2>\n\n\n\n<p><strong>Visit our&nbsp;<a href=\"https:\/\/arrayjet.com\/applications\/reverse-phase-protein-array-rppa\">RPPA application page&nbsp;<\/a>or&nbsp;<a href=\"https:\/\/arrayjet.com\/arrange-consultation\">get in touch<\/a>&nbsp;for a free consultation with our experts.<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Our latest\u00a0application note describes a modified Laemmli buffer formulation and protocol recommendations for RPPA printing on inkjet microarrayers with improved density, spot morphology, and speed.<\/p>\n","protected":false},"author":2,"featured_media":8195,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[55],"tags":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Application Note: Switch your RPPA Assay to Inkjet Printing<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Application Note: Switch your RPPA Assay to Inkjet Printing\" \/>\n<meta property=\"og:description\" content=\"Our latest\u00a0application note describes a modified Laemmli buffer formulation and protocol recommendations for RPPA printing on inkjet microarrayers with improved density, spot morphology, and speed.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing\" \/>\n<meta property=\"og:site_name\" content=\"Arrayjet\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/arrayjet\/\" \/>\n<meta property=\"article:published_time\" content=\"2022-12-12T12:11:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-02-23T12:48:46+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/02\/Mercury-Printing-Nitrocellulose-Blur.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"2100\" \/>\n\t<meta property=\"og:image:height\" content=\"1400\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Ian Livingstone\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@ArrayjetLtd\" \/>\n<meta name=\"twitter:site\" content=\"@ArrayjetLtd\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing#article\",\"isPartOf\":{\"@id\":\"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing\"},\"author\":{\"name\":\"Ian Livingstone\",\"@id\":\"https:\/\/arrayjet.com\/#\/schema\/person\/00c627539bf8e38220f4d9644c42324a\"},\"headline\":\"Application Note: Switch your RPPA Assay to Inkjet Printing\",\"datePublished\":\"2022-12-12T12:11:00+00:00\",\"dateModified\":\"2023-02-23T12:48:46+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing\"},\"wordCount\":144,\"publisher\":{\"@id\":\"https:\/\/arrayjet.com\/#organization\"},\"image\":{\"@id\":\"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing#primaryimage\"},\"thumbnailUrl\":\"https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/02\/Mercury-Printing-Nitrocellulose-Blur.jpg\",\"articleSection\":[\"News\"],\"inLanguage\":\"en-GB\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing\",\"url\":\"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing\",\"name\":\"Application Note: Switch your RPPA Assay to Inkjet Printing\",\"isPartOf\":{\"@id\":\"https:\/\/arrayjet.com\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing#primaryimage\"},\"image\":{\"@id\":\"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing#primaryimage\"},\"thumbnailUrl\":\"https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/02\/Mercury-Printing-Nitrocellulose-Blur.jpg\",\"datePublished\":\"2022-12-12T12:11:00+00:00\",\"dateModified\":\"2023-02-23T12:48:46+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing#breadcrumb\"},\"inLanguage\":\"en-GB\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-GB\",\"@id\":\"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing#primaryimage\",\"url\":\"https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/02\/Mercury-Printing-Nitrocellulose-Blur.jpg\",\"contentUrl\":\"https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/02\/Mercury-Printing-Nitrocellulose-Blur.jpg\",\"width\":2100,\"height\":1400,\"caption\":\"Arrayjet Mercury Printing Nitrocellulose\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/arrayjet.com\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Application Note: Switch your RPPA Assay to Inkjet Printing\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/arrayjet.com\/#website\",\"url\":\"https:\/\/arrayjet.com\/\",\"name\":\"Arrayjet\",\"description\":\"Microarray Printing Instruments, Services &amp; Consumables\",\"publisher\":{\"@id\":\"https:\/\/arrayjet.com\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/arrayjet.com\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"en-GB\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/arrayjet.com\/#organization\",\"name\":\"Arrayjet\",\"url\":\"https:\/\/arrayjet.com\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-GB\",\"@id\":\"https:\/\/arrayjet.com\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/02\/arrayjet-logo-square.jpg\",\"contentUrl\":\"https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/02\/arrayjet-logo-square.jpg\",\"width\":975,\"height\":975,\"caption\":\"Arrayjet\"},\"image\":{\"@id\":\"https:\/\/arrayjet.com\/#\/schema\/logo\/image\/\"},\"sameAs\":[\"https:\/\/www.facebook.com\/arrayjet\/\",\"https:\/\/x.com\/ArrayjetLtd\",\"https:\/\/www.linkedin.com\/company\/arrayjet-ltd\"]},{\"@type\":\"Person\",\"@id\":\"https:\/\/arrayjet.com\/#\/schema\/person\/00c627539bf8e38220f4d9644c42324a\",\"name\":\"Ian Livingstone\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Application Note: Switch your RPPA Assay to Inkjet Printing","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing","og_locale":"en_GB","og_type":"article","og_title":"Application Note: Switch your RPPA Assay to Inkjet Printing","og_description":"Our latest\u00a0application note describes a modified Laemmli buffer formulation and protocol recommendations for RPPA printing on inkjet microarrayers with improved density, spot morphology, and speed.","og_url":"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing","og_site_name":"Arrayjet","article_publisher":"https:\/\/www.facebook.com\/arrayjet\/","article_published_time":"2022-12-12T12:11:00+00:00","article_modified_time":"2023-02-23T12:48:46+00:00","og_image":[{"width":2100,"height":1400,"url":"https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/02\/Mercury-Printing-Nitrocellulose-Blur.jpg","type":"image\/jpeg"}],"author":"Ian Livingstone","twitter_card":"summary_large_image","twitter_creator":"@ArrayjetLtd","twitter_site":"@ArrayjetLtd","schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing#article","isPartOf":{"@id":"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing"},"author":{"name":"Ian Livingstone","@id":"https:\/\/arrayjet.com\/#\/schema\/person\/00c627539bf8e38220f4d9644c42324a"},"headline":"Application Note: Switch your RPPA Assay to Inkjet Printing","datePublished":"2022-12-12T12:11:00+00:00","dateModified":"2023-02-23T12:48:46+00:00","mainEntityOfPage":{"@id":"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing"},"wordCount":144,"publisher":{"@id":"https:\/\/arrayjet.com\/#organization"},"image":{"@id":"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing#primaryimage"},"thumbnailUrl":"https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/02\/Mercury-Printing-Nitrocellulose-Blur.jpg","articleSection":["News"],"inLanguage":"en-GB"},{"@type":"WebPage","@id":"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing","url":"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing","name":"Application Note: Switch your RPPA Assay to Inkjet Printing","isPartOf":{"@id":"https:\/\/arrayjet.com\/#website"},"primaryImageOfPage":{"@id":"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing#primaryimage"},"image":{"@id":"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing#primaryimage"},"thumbnailUrl":"https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/02\/Mercury-Printing-Nitrocellulose-Blur.jpg","datePublished":"2022-12-12T12:11:00+00:00","dateModified":"2023-02-23T12:48:46+00:00","breadcrumb":{"@id":"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing#breadcrumb"},"inLanguage":"en-GB","potentialAction":[{"@type":"ReadAction","target":["https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing"]}]},{"@type":"ImageObject","inLanguage":"en-GB","@id":"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing#primaryimage","url":"https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/02\/Mercury-Printing-Nitrocellulose-Blur.jpg","contentUrl":"https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/02\/Mercury-Printing-Nitrocellulose-Blur.jpg","width":2100,"height":1400,"caption":"Arrayjet Mercury Printing Nitrocellulose"},{"@type":"BreadcrumbList","@id":"https:\/\/arrayjet.com\/switch-rppa-assay-to-inkjet-printing#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/arrayjet.com\/"},{"@type":"ListItem","position":2,"name":"Application Note: Switch your RPPA Assay to Inkjet Printing"}]},{"@type":"WebSite","@id":"https:\/\/arrayjet.com\/#website","url":"https:\/\/arrayjet.com\/","name":"Arrayjet","description":"Microarray Printing Instruments, Services &amp; Consumables","publisher":{"@id":"https:\/\/arrayjet.com\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/arrayjet.com\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"en-GB"},{"@type":"Organization","@id":"https:\/\/arrayjet.com\/#organization","name":"Arrayjet","url":"https:\/\/arrayjet.com\/","logo":{"@type":"ImageObject","inLanguage":"en-GB","@id":"https:\/\/arrayjet.com\/#\/schema\/logo\/image\/","url":"https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/02\/arrayjet-logo-square.jpg","contentUrl":"https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/02\/arrayjet-logo-square.jpg","width":975,"height":975,"caption":"Arrayjet"},"image":{"@id":"https:\/\/arrayjet.com\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/arrayjet\/","https:\/\/x.com\/ArrayjetLtd","https:\/\/www.linkedin.com\/company\/arrayjet-ltd"]},{"@type":"Person","@id":"https:\/\/arrayjet.com\/#\/schema\/person\/00c627539bf8e38220f4d9644c42324a","name":"Ian Livingstone"}]}},"_links":{"self":[{"href":"https:\/\/arrayjet.com\/wp-json\/wp\/v2\/posts\/8193"}],"collection":[{"href":"https:\/\/arrayjet.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/arrayjet.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/arrayjet.com\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/arrayjet.com\/wp-json\/wp\/v2\/comments?post=8193"}],"version-history":[{"count":3,"href":"https:\/\/arrayjet.com\/wp-json\/wp\/v2\/posts\/8193\/revisions"}],"predecessor-version":[{"id":8198,"href":"https:\/\/arrayjet.com\/wp-json\/wp\/v2\/posts\/8193\/revisions\/8198"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/arrayjet.com\/wp-json\/wp\/v2\/media\/8195"}],"wp:attachment":[{"href":"https:\/\/arrayjet.com\/wp-json\/wp\/v2\/media?parent=8193"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/arrayjet.com\/wp-json\/wp\/v2\/categories?post=8193"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/arrayjet.com\/wp-json\/wp\/v2\/tags?post=8193"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}