{"id":9060,"date":"2023-06-21T20:10:36","date_gmt":"2023-06-21T19:10:36","guid":{"rendered":"https:\/\/arrayjet.com\/?p=9060"},"modified":"2024-02-02T13:25:32","modified_gmt":"2024-02-02T13:25:32","slug":"screening-correctly-folded-gpcrs-and-other-important-targets-using-membrane-embedded-protein-arrays","status":"publish","type":"post","link":"https:\/\/arrayjet.com\/screening-correctly-folded-gpcrs-and-other-important-targets-using-membrane-embedded-protein-arrays","title":{"rendered":"Screening correctly-folded GPCRs and other important targets using membrane-embedded protein arrays."},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"9060\" class=\"elementor elementor-9060\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-7c4914b2 e-flex e-con-boxed e-con e-parent\" data-id=\"7c4914b2\" data-element_type=\"container\" data-core-v316-plus=\"true\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-23b7c3c8 elementor-widget elementor-widget-text-editor\" data-id=\"23b7c3c8\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.20.0 - 26-03-2024 *\/\n.elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#69727d;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#69727d;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}<\/style>\t\t\t\t<!-- wp:heading -->\n<h2 class=\"wp-block-heading\"><a href=\"https:\/\/www.cdilabs.com\/products\/vird-membrane-protein-array\" target=\"_blank\" rel=\"noopener\">Virion Membrane Display (VirD\u2122)<\/a> microarrays from CDI Laboratories allow high-throughput screening against functional human membrane proteins.<\/h2>\n<!-- \/wp:heading --><!-- wp:spacer {\"height\":\"20px\"} -->\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\n<!-- \/wp:spacer --><!-- wp:paragraph -->\n<p>CDI Laboratories is a long-time partner of Arrayjet with an extensive history in protein arrays. They\u2019ve produced their highly impactful <a href=\"https:\/\/www.cdilabs.com\/products\/huprot-microarray\" target=\"_blank\" rel=\"noopener\">HuProt\u2122 Human Proteome Array<\/a> with Arrayjet printers since 2016. With their newest platform, they focus on membrane proteins.<\/p>\n<!-- \/wp:paragraph --><!-- wp:paragraph -->\n<p>Many therapeutically relevant proteins are membrane proteins, which can be difficult to express in a functional conformation for laboratory research. CDI\u2019s new <a href=\"https:\/\/www.cdilabs.com\/products\/vird-membrane-protein-array\" target=\"_blank\" rel=\"noopener\">Virion Membrane Display (VirD\u2122) <\/a>platform can produce panels of these proteins that retain proper folding and biochemical activity (Syu et al. 2019).<\/p>\n<!-- \/wp:paragraph --><!-- wp:spacer {\"height\":\"20px\"} -->\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\n<!-- \/wp:spacer --><!-- wp:quote {\"style\":{\"typography\":{\"fontStyle\":\"normal\",\"fontWeight\":\"500\",\"fontSize\":\"16px\"}}} -->\n<blockquote class=\"wp-block-quote\" style=\"font-size: 16px; font-style: normal; font-weight: 500;\"><!-- wp:paragraph {\"style\":{\"typography\":{\"fontSize\":\"16px\",\"fontStyle\":\"normal\",\"fontWeight\":\"400\"}}} -->\n<p style=\"font-size: 16px; font-style: normal; font-weight: 400;\"><em>\u201cWe&#8217;re excited to present VirD\u2122 as a new option for screening ligands and binders against difficult, drug-relevant targets such as GPCRs, immune checkpoints, and neuronal receptors. The precision of the Arrayjet microarrayers help us print the highest quality microarrays.&#8221;<\/em><\/p>\n<!-- \/wp:paragraph --><cite>Scott D. Paschke, Vice President at CDI Laboratories<\/cite><\/blockquote>\n<!-- \/wp:quote --><!-- wp:spacer {\"height\":\"20px\"} -->\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\n<!-- \/wp:spacer --><!-- wp:image {\"id\":9064,\"width\":840,\"height\":337,\"sizeSlug\":\"large\",\"linkDestination\":\"media\"} -->\n<figure class=\"wp-block-image size-large is-resized\"><a href=\"https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/06\/CDI-VirD-Membrane-Protein-Microarray-Library-Creation-Validation-and-Production.png\"><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-9064\" src=\"https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/06\/CDI-VirD-Membrane-Protein-Microarray-Library-Creation-Validation-and-Production-1024x411.png\" alt=\"Overview of VirD validation and production.\" width=\"840\" height=\"337\" srcset=\"https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/06\/CDI-VirD-Membrane-Protein-Microarray-Library-Creation-Validation-and-Production-1024x411.png 1024w, https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/06\/CDI-VirD-Membrane-Protein-Microarray-Library-Creation-Validation-and-Production-300x120.png 300w, https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/06\/CDI-VirD-Membrane-Protein-Microarray-Library-Creation-Validation-and-Production-768x308.png 768w, https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/06\/CDI-VirD-Membrane-Protein-Microarray-Library-Creation-Validation-and-Production-1536x616.png 1536w, https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/06\/CDI-VirD-Membrane-Protein-Microarray-Library-Creation-Validation-and-Production-2048x822.png 2048w, https:\/\/arrayjet.com\/wp-content\/uploads\/2023\/06\/CDI-VirD-Membrane-Protein-Microarray-Library-Creation-Validation-and-Production-600x241.png 600w\" sizes=\"(max-width: 840px) 100vw, 840px\" \/><\/a>\n<figcaption class=\"wp-element-caption\"><em>VirD\u2122 Validation and Production. Image courtesy of CDI Laboratories. Click to expand.<\/em><\/figcaption>\n<\/figure>\n<!-- \/wp:image --><!-- wp:spacer {\"height\":\"20px\"} -->\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\n<!-- \/wp:spacer --><!-- wp:paragraph -->\n<p>In VirD\u2122, human cells are infected with engineered HSV virions, which leverage the host expression system to display a natively folded membrane protein on the viral envelope.<\/p>\n<!-- \/wp:paragraph --><!-- wp:paragraph -->\n<p>Arrayjet microarrayers are used to print successfully transfected virions into an array for convenient screening. The current VirD\u2122 v2.0 GPCR Array contains 259 non-odorant GPCR proteins and 97 miscellaneous (non-GPCR) membrane proteins. In future, a comprehensive array of 6000+ membrane proteins may be possible.<\/p>\n<!-- \/wp:paragraph --><!-- wp:spacer {\"height\":\"20px\"} -->\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\n<!-- \/wp:spacer --><!-- wp:quote {\"style\":{\"typography\":{\"fontSize\":\"16px\",\"fontStyle\":\"normal\",\"fontWeight\":\"500\"}}} -->\n<blockquote class=\"wp-block-quote\" style=\"font-size: 16px; font-style: normal; font-weight: 500;\"><!-- wp:paragraph {\"style\":{\"typography\":{\"fontStyle\":\"normal\",\"fontWeight\":\"400\",\"fontSize\":\"16px\"}}} -->\n<p style=\"font-size: 16px; font-style: normal; font-weight: 400;\"><em>\u201cWe have seen growing interest in the study of transmembrane proteins and CDI\u2019s new VirD\u2122 array will be game changing,\u00a0as it harnesses the power of array-based screening to integrate this high-impact protein family. I look forward to the exciting new biology they will discover.\u201c<\/em><\/p>\n<!-- \/wp:paragraph --><cite>Adam Buckle, CSO at Arrayjet<\/cite><\/blockquote>\n<!-- \/wp:quote --><!-- wp:spacer {\"height\":\"20px\"} -->\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\n<!-- \/wp:spacer --><!-- wp:quote {\"style\":{\"typography\":{\"fontSize\":\"16px\",\"fontStyle\":\"normal\",\"fontWeight\":\"500\"}}} -->\n<blockquote class=\"wp-block-quote\" style=\"font-size: 16px; font-style: normal; font-weight: 500;\"><!-- wp:paragraph {\"style\":{\"typography\":{\"fontSize\":\"16px\",\"fontStyle\":\"normal\",\"fontWeight\":\"400\"}}} -->\n<p style=\"font-size: 16px; font-style: normal; font-weight: 400;\"><em><em>\u201cIt\u2019s always a pleasure to see the innovations from our community of instrument users. With this platform CDI is addressing an important research challenge and we\u2019re delighted to continue to support them.\u201d<\/em><\/em><\/p>\n<!-- \/wp:paragraph --><cite>Iain McWilliam, CEO at Arrayjet<\/cite><\/blockquote>\n<!-- \/wp:quote --><!-- wp:spacer {\"height\":\"20px\"} -->\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\n<!-- \/wp:spacer --><!-- wp:paragraph {\"style\":{\"typography\":{\"fontStyle\":\"normal\",\"fontWeight\":\"500\"}}} -->\n<p style=\"font-style: normal; font-weight: 500;\"><a href=\"https:\/\/www.cdilabs.com\/products\/vird-membrane-protein-array\" target=\"_blank\" rel=\"noopener\"><strong>Visit CDI\u2019s website to learn more about VirD\u2122 array specifications, assay method, and analysis \u00bb<\/strong><\/a><\/p>\n<!-- \/wp:paragraph --><!-- wp:spacer {\"height\":\"20px\"} -->\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\n<!-- \/wp:spacer --><!-- wp:heading {\"level\":6} -->\n<h6 class=\"wp-block-heading\">More from CDI and Arrayjet<\/h6>\n<!-- \/wp:heading --><!-- wp:list -->\n<ul><!-- wp:list-item -->\n<li><a href=\"https:\/\/arrayjet.com\/webinars\/seromics-decoding-the-antibody-fingerprint\">Webinar \u00bb Seromics: Decoding the Antibody Fingerprint<\/a><\/li>\n<!-- \/wp:list-item --><!-- wp:list-item -->\n<li><a href=\"https:\/\/arrayjet.com\/case-studies\/cdi-laboratories-the-largest-human-proteome-array-huprot\">Case Study \u00bb CDI Laboratories: the largest Human Proteome Array (HuProt\u2122)<\/a><\/li>\n<!-- \/wp:list-item --><\/ul>\n<!-- \/wp:list --><!-- wp:spacer {\"height\":\"20px\"} -->\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\n<!-- \/wp:spacer --><!-- wp:heading {\"level\":6} -->\n<h6 class=\"wp-block-heading\">VirD\u2122 Publications<\/h6>\n<!-- \/wp:heading --><!-- wp:paragraph {\"fontSize\":\"small\"} -->\n<p class=\"has-small-font-size\">S. Hu, Y. Feng, B. Henson, B. Wang, X. Huang, M. Li, P. Desai, H. Zhu, VirD: A Virion Display Array for Profiling Functional Membrane Proteins, Anal. Chem. 85 (2013) 8046\u20138054. <a href=\"https:\/\/doi.org\/10.1021\/ac401795y\">https:\/\/doi.org\/10.1021\/ac401795y<\/a><\/p>\n<!-- \/wp:paragraph --><!-- wp:paragraph {\"fontSize\":\"small\"} -->\n<p class=\"has-small-font-size\">G.-D. Syu, S.-C. Wang, G. Ma, S. Liu, D. Pearce, A. Prakash, B. Henson, L.-C. Weng, D. Ghosh, P. Ramos, D. Eichinger, I. Pino, X. Dong, J. Xiao, S. Wang, N. Tao, K.S. Kim, P.J. Desai, H. Zhu, Development and application of a high-content virion display human GPCR array, Nat Commun. 10 (2019) 1997. <a href=\"https:\/\/doi.org\/10.1038\/s41467-019-09938-9\">https:\/\/doi.org\/10.1038\/s41467-019-09938-9<\/a><\/p>\n<!-- \/wp:paragraph -->\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Virion Membrane Display (VirD\u2122) microarrays from CDI Laboratories allow high-throughput screening against functional human membrane proteins.<\/p>\n","protected":false},"author":6,"featured_media":9062,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[28],"tags":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Screening correctly-folded GPCRs and other important targets using membrane-embedded 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