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PolyAn NHS

PolyAn 3-D NHS (25 pack)

£287

SKU AJC2009 Category Tag

Product Code  104 00 401

Product Description

Arrayjet offers a broad PolyAn product portfolio including plastic and glass slides as well as functionalized 96-well plates for DNA, peptide or protein applications ranging from low-density microarrays and diagnostic tests to ultra-dense arrays for high-throughput screening.

PolyAn 3D-NHS slides are manufactured from high-quality glass with an ultra-flat surface and low background fluorescence.  The glass is coated with a thin NHS-ester layer that will covalently bind most biomolecules.

The slides are easy to work with and are fully compatible with all Arrayjet microarray printers and all slide scanners.

Technical Info

The NHS-ester reacts immediately with the NH2-terminus of biochemical species to form a covalent bond with the surface (420 kJ/mol).

The reaction of carboxyl groups with N-Hydroxy succinimide leads to highly reactive esters, which can be easily reacted with nucleophiles e.g. Amines, Hydrazines. However, due to its high reactivity the NHS-ester is susceptible against hydrolysis and is characterized by a relatively short shelf-life. All NHS-activated surfaces should therefore be processed quickly.

There are a number of different approaches to couple on the NHS surface:

• It is assumed that not all Carboxy groups have reacted to NHS-esters during activation. Thus a negatively charged carboxy surface still remains which in turn supports the physico-chemical adsorption of positively charged probes e.g. NH3. Hence a protonating media (pH < 5) for the biochemical species to get a positive charge is required.

• A nucleophilic attack on the active ester is also catalysed under basic conditions (pH > 8.5). After attachment of the biochemical species the surfaces must be blocked with a blocking buffer containing small molecules that can access all reactive groups within the 3D-Matrix.

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Microarray Instruments & Services

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