Application Note

Technology feature: Inkjet printing for the production of protein microarrays

In this chapter for Methods in Molecular Biology, we present our core inkjet technology for the printing protein arrays.

The ability of Arrayjet technology to print viscous samples in viscous cryoprotectant solutions such as glycerol or ethylene glycol is a distinct advantage.  Our contactless, on-the-fly sample deposition is ideal for printing protein microarrays on delicate nitrocellulose or hydrogel surfaces.

By providing the most high-throughput technology, Arrayjet enables researchers to quickly produce multiplex arrays for commercial screening, validation or diagnostic purposes.


A significant proportion of protein microarray researchers would like the arrays they develop to become widely used research, screening, validation or diagnostic devices. For this to be achievable the arrays must be compatible with high-throughput techniques that allow manufacturing scale production. In order to simplify the transition from laboratory bench to market, Arrayjet have developed a range of inkjet microarray printers, which, at one end of the scale, are suitable for R&D and, at the other end, are capable of true high-throughput array output. To maintain scalability, all Arrayjet microarray printers utilise identical core technology comprising a JetSpyder™ liquid handling adaptor, which enables automated loading of an industry-standard inkjet printhead compatible with non-contact on-the-fly printing. This chapter contains a detailed explanation of Arrayjet technology followed by a historical look at the development of inkjet technologies for protein microarray production. The method described subsequently is a simple example of an antibody array printed onto nitrocellulose-coated slides with specific detection with fluorescently labelled IgG. The method is linked to a notes section with advice on best practice and sources of useful information for protein microarray production using inkjet technology.

Citation information:

McWilliam I, Chong Kwan M, Hall D. Inkjet printing for the production of protein microarrays. Methods Mol Biol. 2011;785:345-61. doi: 10.1007/978-1-61779-286-1_23. PMID: 21901611.

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