Arrayjet
Application Note

RPPA: Transition from contact pin spotting of cell lysates and protein extracts to high-speed, contactless inkjet printing

Modification of Laemmli buffer formulation for Reverse-Phase Protein Array (RPPA) using Arrayjet inkjet microarrayers.

Many research groups print microarrays of cell lysates and protein extracts for Reverse Phase Protein Array (RPPA). This application note describes a modified Laemmli buffer formulation and protocol recommendations for RPPA printing on inkjet microarrayers with improved density, spot morphology, and speed.

It is intended as a basic guide for researchers and technicians considering moving their RPPA printing procedures from contact pin spotting to inkjet microarray technology.

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