Introduction
This application note highlights the successful printing of fluorescent-tagged antibodies onto Schott Nexterion® epoxysilane slides using Arrayjet JetStar™ Protein Printing Buffer C to produce high-quality spot morphology. It provides evidence of the consistency and reproducibility of antibody arrays manufactured on Arrayjet microarrayers.
Printing buffer plays a crucial part when designing a protein array experiment. To achieve high quality spot morphology, targets to be printed using Arrayjet Inkjet technology should have a viscosity between 4-20 centipoise (cP). At Arrayjet we have optimised a range of printing buffers to print different sample types. Initially each printing buffer should be evaluated for each type of protein to achieve good spot morphology without affecting the protein functionality. We achieved high quality spots when printing Immunoglobulin-G (IgG) using JetStar™ Protein Printing Buffer C.
The print head delivers 100 pL droplets with a maximum delivery volume of 10 nL achieved by delivering multiple drops per spot. One drop produces spots of approximately 100 μm diameter. By increasing the number of drops per spot during printing the spot diameter can be controlled. However, the spot diameter is also dependent on factors such as temperature, humidity, the printing buffer and the surface chemistry of the slide.
